Journal:

Article Title: Enrichment for murine keratinocyte stem cells based on cell surface phenotype

doi:

Figure Lengend Snippet: mAb 10G7 recognizes the human transferrin receptor (HTR) CD71. Flow cytometric analysis of CEF + HTR cells showing their reactivity with a known anti-CD71 antibody (B3/25.4) and mAb 10G7. mAb 1D4.5 is an isotype-matched negative control for mAb 10G7.

Article Snippet: Cells were processed for two-color staining for α 6 and CD71 for FACS by incubating with mAb GoH3 (anti-α 6 integrin rat monoclonal; IgG 2a ) at 10 μg/ml (Serotec), and mAb R17 208.2 (a rat anti-mouse CD71 IgM, provided by Bob Hyman, Salk Institute) used as hybridoma supernatant for 1 h, after blocking in 4-ml blocking buffer (4% normal human serum/0.4% BSA/5% FCS in HBSS) for 20 min at 4°C.

Techniques: Negative Control

Journal:

Article Title: Enrichment for murine keratinocyte stem cells based on cell surface phenotype

doi:

Figure Lengend Snippet: Two-color flow cytometric analysis of α6 integrin and CD71 expression on primary murine dorsal keratinocytes. α6 was detected with FITC (x axis, FL1) and CD71 with phycoerythrin (y axis, FL2). Three phenotypically distinct fractions of α6 positive keratinocytes were consistently discernable (n = 25) as indicated: a, α6briCD71bri cells making up the majority of basal keratinocytes; b, α6briCD71dim cells representing a discrete but minor proportion of basal keratinocytes; and c, α6dim cells that appear as a less discrete population. A number of α6 negative nonepithelial cells were also detected.

Article Snippet: Cells were processed for two-color staining for α 6 and CD71 for FACS by incubating with mAb GoH3 (anti-α 6 integrin rat monoclonal; IgG 2a ) at 10 μg/ml (Serotec), and mAb R17 208.2 (a rat anti-mouse CD71 IgM, provided by Bob Hyman, Salk Institute) used as hybridoma supernatant for 1 h, after blocking in 4-ml blocking buffer (4% normal human serum/0.4% BSA/5% FCS in HBSS) for 20 min at 4°C.

Techniques: Expressing

Journal:

Article Title: Enrichment for murine keratinocyte stem cells based on cell surface phenotype

doi:

Figure Lengend Snippet: Cell size and nuclear and cytoplasmic areas of fractionated keratinocytes

Article Snippet: Cells were processed for two-color staining for α 6 and CD71 for FACS by incubating with mAb GoH3 (anti-α 6 integrin rat monoclonal; IgG 2a ) at 10 μg/ml (Serotec), and mAb R17 208.2 (a rat anti-mouse CD71 IgM, provided by Bob Hyman, Salk Institute) used as hybridoma supernatant for 1 h, after blocking in 4-ml blocking buffer (4% normal human serum/0.4% BSA/5% FCS in HBSS) for 20 min at 4°C.

Techniques:

Journal:

Article Title: Enrichment for murine keratinocyte stem cells based on cell surface phenotype

doi:

Figure Lengend Snippet: Distribution of LRCs and PLCs in primary fractionated keratinocytes

Article Snippet: Cells were processed for two-color staining for α 6 and CD71 for FACS by incubating with mAb GoH3 (anti-α 6 integrin rat monoclonal; IgG 2a ) at 10 μg/ml (Serotec), and mAb R17 208.2 (a rat anti-mouse CD71 IgM, provided by Bob Hyman, Salk Institute) used as hybridoma supernatant for 1 h, after blocking in 4-ml blocking buffer (4% normal human serum/0.4% BSA/5% FCS in HBSS) for 20 min at 4°C.

Techniques:

Journal:

Article Title: Enrichment for murine keratinocyte stem cells based on cell surface phenotype

doi:

Figure Lengend Snippet: The bulge region of the hair follicle is CD71dim. (a) Immunofluorescence micrographs of CD71 staining (green) in dorsal skin illustrating several early anagen hair follicles (arrowheads), with bright staining for CD71 at the base of the follicle. Note the longitudinal section through a mid-anagen hair follicle showing relatively low to negative CD71 expression in the bulge region (block arrows), directly below the sebaceous glands (arrow). (b and c) Dual immunofluorescence for CD71 (green) and nuclei stained with propidium iodide (red) illustrating the presence of CD71dim cells in the bulge region (b) and CD71bri cells in the bulb region (c). Dermal papilla is indicated by the star.

Article Snippet: Cells were processed for two-color staining for α 6 and CD71 for FACS by incubating with mAb GoH3 (anti-α 6 integrin rat monoclonal; IgG 2a ) at 10 μg/ml (Serotec), and mAb R17 208.2 (a rat anti-mouse CD71 IgM, provided by Bob Hyman, Salk Institute) used as hybridoma supernatant for 1 h, after blocking in 4-ml blocking buffer (4% normal human serum/0.4% BSA/5% FCS in HBSS) for 20 min at 4°C.

Techniques: Immunofluorescence, Staining, Expressing, Blocking Assay